1、山东大学分子生物学相关资料Section A - Cells and macromolecules 1The glycosylation of secreted proteins takes place in the . . .A mitochondria. B peroxisomes. C endoplasmic reticulum. D nucleus. 2Which of the following is an example of a nucleoprotein?A keratin. B chromatin. C histone. D proteoglycan.3Which of th
2、e following is not a polysaccharide? A chitin. B amylopectin. C glycosaminoglycan.D glycerol. 4. Transmembrane proteinsA join two lipid bilayers together. B have intra- and extracellular domains. C are contained completely within the membrane. D are easily removed from the membrane.Section B - Prote
3、in structure1. Which of the following is an imino acid? A proline. B hydroxy lysine. C tryptophan. D histidine. 2 Protein family members in different species that carry out the same biochemical role are described as . . . A paralogs. B structural analogs. C heterologs. D orthologs. 3. Which of the f
4、ollowing is not a protein secondary structure?A -helix.B triple helix. C double helix. D -pleated sheet. 4 In isoelectric focusing, proteins are separated .A in a pH gradient. B in a salt gradient. C in a density gradient. D in a temperature gradient.5Edman degradation sequences peptides . . . A usi
5、ng a cDNA sequence. B according to their masses. C From the C-terminus to the N-terminus. D from the N-terminus to the C-terminus. Section C - properties of nucleic acids1The sequence 5-AGTCTGACT-3 in DNA is equivalent to which sequence in RNA? A 5-AGUCUGUGACU -3 B 5 -UGTCTGUTC -3 C 5 -UCAGUCUGA-3 D
6、 5- AGUCAGACU-3 2. Which of the following correctly describes A-DNA? A a right-handed antiparallel double helix with 10 bp/turn and bases lying perpendicular to the helix axis. B a left-handed antiparallel double-helix with 12 bp/turn formed from alternating pyrimidine-purine sequences. C a right-ha
7、nded antiparallel double helix with 11 bp/turn and bases tilted with respect to the helix axis. D a globular structure formed by short intramolecular helices formed in a single-strand nucleic acid. 3. Denaturation of double stranded DNA involves.A preakage into short double-stranded fragments.B sepa
8、ration into single strands. C hydrolysis of the DNA backbone.D cleavage of the bases from the sugar-phosphate backbone. 4. Which has the highest absorption per unit mass at a wavelength of 260 nm?A double-stranded DNA. B mononucleotides. C RNA. D protein. 5. Type I DNA topoisomeraes .A change linkin
9、g number by士2 B require ATP. C break one strand of a DNA double helix. D are the target of antibacterial drugs. Section D - Prokaryotic and eukaryotic chromatin structure1Which of the following is common to both E. coli and eukaryotic chromosomes? A the DNA is circular. B the DNA is packaged into nu
10、cleosomes.C the DNA is contained in the nucleus. D the DNA is negatively supercoiled. 2A compl of 166 bp of DNA with the histone octamer plus histone HI is known as a . . . A nucleosome core. B solenoid. C 30 nm fiber. D chromatosome. 3In what region of the interphase chromosome does transcription t
11、ake place? A the telomere. B the centromere. C euchromatin. D heterochromatin. 4 Which statement about CpG islands and methylation is not true? A CpG islands are particularly resistant to DNase I. B CpG methylation is responsible for the mutation of CpG to TpG in eukaryotes. C CpG islands occur arou
12、nd the promoters of active genes. D CpG methylation is associated with inactive chromatin. 5Which of the following is an example of highly-repetitive DNA? A Alu element. B histone gene cluster. C DNA minisatellites. D dispersed repetitive DNA. Section E - DNA replication1The number of replicons in a
13、 typical mammalian cell is . . . A 40-200. B 400. C 1000-2000. D 50000-100000. 2. In prokaryotes,the lagging strand primers are removed by . . . A 3 to 5 exonuclease. B DNA ligase. C DNA polymerase I. D DNA polymerase III. 3. The essential initiator protein at the E. coli origin of replication is .
14、. . A DnaA. B DnaB. C DnaC. D DnaE. 4. Which phase would a cell enter if it was starved of mitogens before the R point? A G1. B S. C G2. D G0. 5. Which one of the following statements is true? A once the cell has passed the R point, cell division is inevitable. B the phosphorylation of Rb by a G1 cy
15、clin-CDK complex is a critical requirement for entry into S phase . C phosphorylation of E2F by a G1 cyclin-CDK complex is a critical requirement for entry into S phase. D cyclin D1 and INK4 p16 are tumor suppressor proteins. 6. In eukaryotes, euchromatin replicates predominantly. A in early S-phase
16、. B in mid S-phase. C in late S-phase. D in G2-phase. 7. Prokaryotic plasmids can replicate in yeast cells if they contain a cloned yeast. . . A ORC. B CDK. C ARS. D RNA. Section F - DNA damage, repair and recombination1. Per nucleotide incorporated, the spontaneous mutation frequency in E. coli is
17、. . . A 1 in 106. B 1 in 108. C 1 in 109. D 1 in 1010. 2. The action of hydroxyl radicals on DNA generates a significant amount of . . . A pyrimidine dimmers.B 8-oxoguanine.C O6- methylguanine. D 7-hydroxymethylguanine.3. In methyl-directed mismatch repair in E. coli, the daughter strand containing
18、the mismatched base is nicked by . . . AMutH endonuclease.BUvrABC endonuclease.CAP endonuclease.D3 to 5 exonuclease.4. Illegitimate recombination is another name for . . . A site-specific recombination. B transposition.C homologous recombination.D translesion DNA synthesis. 5. The excision repair of
19、 UV-induced DNA damage is defective in individuals suffering from . A hereditary nonpolyposis colon cancer. B Crohns disease. C classical xeroderma pigmentosum. D xeroderma pigmentosum variant. Section G - Gene manipulation1.The presence of a plasmid in a bacterial culture is usually determined by .
20、 . . A blue-white screening. B growth in the presence of an antibiotic. C a restriction enzyme digest. D agarose gel electrophoresis. 2.The enzyme alkaline phosphatase. . . A the take-up of a plasmid into a bacterium. B the expression of a gene in a bacterium.C the take-up of a bacteriophage into a
21、bacterium.D the isolation of a plasmid from a bacterium. 3.Transformation is . . . A the take-up of a plasmid into a bacterium. B the expression of a gene in a bacterium.、 C the take-up of a bacteriophage into a bacterium. D the isolation of a plasmid from a bacterium. 4. T4 DNA ligase . . . A requi
22、res ATP. B joins double-stranded DNA fragments with an adjacent 3-phosphate and 5-OH. C requires NADH. D joins single-stranded DNA.5. In agarose gel electrophoresis . . . A DNA migrates towards the negative electrode. B supercoiled plamids migrate slower than their nicked counterparts. C larger mole
23、cules migrate faster than smaller molecules.D ethidium bromide can be used to visualize the DNA. Section H - Cloning vectors 1. Blue-white selection is used. . . A to test for the presence of a plasmid in bacteria. B to reveal the identity of a cloned DNA fragment. C to express the product of a clon
24、ed gene. D to test for the presence of a cloned insert in a plasmid. 2. A multiple cloning site . . . A contains many copies of a cloned gene. B allows flexibility in the choice of restriction enzymes for cloning. C allows flexibility in the choice of organism for cloning. D contains many copies of
25、the same restriction enzyme site. 3. Infection of E. coli by bacteriophage is normally detected by . . . A resistance of the bacteria to an antibiotic. B the growth of single bacterial colonies on an agar plate. C the appearance of areas of lysed bacteria on an agar plate. D restriction digest of th
26、e bacterial DNA. 4. Which vector would be most appropriate for cloning a 150 kb fragment of DNA? A a plasmid. B a vector. C a BAC. D a YAC. 5. Which vector would you chqose to express a foreign gene in a plant? A a baculovirus vector. B a retroviral vector. C a Yep vector. D a T-DNA vector. Section
27、I - Gene libraries and screening 1.Which two of the following statements about genomic libraries are false? A genomic libraries are made from cDNA. B genomic libraries must be representative if they are to contain all the genes in an organism. C genomic libraries must contain a minimum number of rec
28、ombinants if they are to contain all the genes In an orgamsm. D the DNA must be fragmented to an appropriate size for the vector that is used. E genomic libraries made from eukaryotic DNA usually use plasmid vectors. 2.Which statement correctly describes sequential steps in cDNA cloning?A reverse tr
29、anscription of Mrna second strand synthesis cDNA end modification ligation to vector. B mRNA preparation cDNA synthesis using reverse transcriptase second strand synthesis using terminal transferase, ligation to vector. C mRNA synthesis using RNA polymerase reverse transcription of mRNA, second stra
30、nd synthesis, ligation to vector. D double stranded cDNA synthesis restriction enzyme digestion addition of linkers ligation to vector. 3. Which one of the following is not a valid method of screening a library? A hybridization of colony / plaque-lifted DNA using a nucleic acid probe. B using antibo
31、dies raised against the protein of interest to screen an expression library. C screening pools of clones from an expression library for biological activity. D hybridization of colony/plaque-lifted DNA using an antibody probe. Section J - Analysis and uses of cloned DNA 1.A linear DNA fragment is (10
