硕士生物仪器分析之气相色谱.ppt
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硕士生物仪器分析之气相色谱.ppt
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气相色谱GasChromatography(GC)简介简介特点特点基本基本理论理论仪器流程及主要部件仪器流程及主要部件应用应用发展趋势发展趋势ChromatographyIsatechniqueusedtoseparateandidentifythecomponentsofamixture.Worksbyallowingthemoleculespresentinthemixturetodistributethemselvesbetweenastationaryandamobilemedium.Moleculesthatspendmostoftheirtimeinthemobilephasearecarriedalongfaster.IntroductionGasChromatographyHerethemobilephaseisanunreactivegas(egNitrogen)flowingthroughatube.Andthestationaryphaseisaninvolatileliquidheldonparticlesofasolidsupport.IntroductionGasChromatography(GC)isnowamatureanalyticaltoolparticularlyusefulintheanalysisofvolatileandthermallystableanalytes.Characteristic气相色谱可用来分析能能瞬瞬间间气气化化且且不不分分解解的、的、沸点0.1%)GCanalysisoffragrances格拉斯格拉斯(Grasse)世界香水之都世界香水之都提问时间FutureTrendsintheSeparationSciences1.MiniaturizationComparisonamongGCcolumns(inscale)ComparisonamongHPLCcolumns(inscale)packedcolumnscapillarycolumns2.Neithertemperature,norpressure:
electricalfieldGCthecolumntemperatureHPLCapressuregradientsolventselectrodrivenelectrophoretictechniques(hybridtechniquesthatuseaspectsofchromatographyaswellasaspectsofelectrophoresis)FutureTrendsintheSeparationSciences3.HyphenatedsystemsLC-LC(suchas:
aRP-18column+achiralcolumn)SFE-CESFE-SPE-HRGCLC-GCSPME-GC-MSSFE-SFCFutureTrendsintheSeparationSciences4.TheroleofthesolventinseparationsciencesFutureTrendsintheSeparationScienceshttp:
/www.scielo.br/scielo.php?
script=sci_arttext&pid=S0103-505320030002000055.Highspeedseparationtechniques6.Automationoftheseparationtechniques7.UnifiedchromatographyFutureTrendsintheSeparationSciencesGeneralseparationapproachemployedinatypicalcomplexanalysis1.ExtractionofsolidsamplesExtractionTechniquesSoxhletextractionMicrowaveextractionSonicationandsimilartechniquesPressurizedsolventtechniqueSupercriticalfluidextraction(SFE)Subcriticalwaterextraction(SWE)Acceleratedsolventextraction(ASE)2.ExtractionofliquidsamplesLiquid-liquidextraction(LLE)Solid-phaseextraction(SPE)Solidphasemicroextraction(SPME)Stirbarsorptiveextraction(SBSE)Pressurizedsolventextraction(PSE)SFESWEASEExtractionTechniques3.ExtractionofgaseoussamplesHeadspacetechniquethestaticheadspace(SH)approachesthedynamicheadspace(DH)approachesHeadspace-Solidphasemicroextraction(HS-SPME)ExtractionTechniques
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- 硕士 生物 仪器 分析 色谱